Ca-EDTA知見補遺 : 2 pbと結合した赤血球及びpbを除去した赤血球の変化について

Abstract

Studies on combination of erythrocytes with lead were made physically or chemically through hemolysis. Normal erythrocytes (A), erythrocytes bound with lead (B), and erythrocytes whose bound lead was removed with Ca-EDTA (C) were respectively hemolysed by the use of hypotonic saline solution, saponin, monoiodacetic acid, heat, and ultrasonic wave for comparative studies of their behavior to the hemolytic factors. (A) consisted of normal human erythrocytes rinsed with physiological saline solution and suspended in physiological saline solution at a rate of 2%; (B) was prepared by putting 5×10-4 M of Pb into (A); and (C) was obtained by adding into (B) Ca-EDTA twice the equivalent of Pb. 1) Resistance to hypotonic saline solution was the highest in (A), followed in order by (C) and (B). 2) Resistance to saponin was the highest in (A), followed in order by (B) and (C). As a result of the removal of Pb, the recovery of resistance was not observed. 3) Erythrocytes bound with lead showed remarkably increased resistance to the hemolytic activity of monoiodacetic acid; and the resistance was lost following the removal of Pb. 4) Erythrocytes bound withlead as well as Pb-freed erythrocytes were more apt to undergo hemolysis by heat. 5) Hemolysis by ultrasonic wave showed no difference among three. The above findings suggest that Pb has the greatest effect upon the blood cell membrane. The results of the experiments with saponin and monoiodacetic acid indicate that the combination between Pb and blood cell membrane is a chemical phenomenon, suggesting the presence of a close relationship of Pb with lipoid and SH groups of protein in the cell menbrane. The combination velocity of Pb with erythrocytes is very high. However, the effect of the above binding does not appear so soon, and becomes increasingly noted with the lapse of time.