Ca 2+-oscillations and Ca 2+-waves in mammalian cardiac and vascular smooth muscle cells

Abstract

In this article, we review briefly the available theories and data on [Ca 2+] i-waves and [Ca 2+] i-oscillations in mammalian cardiac and vascular smooth muscle. In addition to our review, we also report: (i) the existence and characterization of rapid agonist-induced [Ca 2+] i-waves in cultured vascular smooth muscle cells (A7r5 cells); and (ii) a new method for studying rapid [Ca 2+] i-waves in mammalian cardiac ventricular cells. In mammalian cardiac muscle several types of Ca 2+-release from sarcoplasmic reticulum (SR) are known to occur and might be involved in Ca 2+-waves and Ca 2+-oscillations: (a) Ca 2+-induced release of Ca 2+, of the type thought to be important in normal excitation-contraction coupling; (b) spontaneous, cyclic release of Ca 2+ related to a Ca 2+-overload of the SR; and (c) Ins(1,4,5)P 3-induced Ca 2+-release. The available data support the idea that [Ca 2+] i-waves in heart propagate by a mechanism somewhat different than that involved in normal excitation-contraction coupling (a, above), perhaps involving spontaneous release of Ca 2+ from an overloaded SR (b, above). In mammalian vascular smooth muscle, our data support the idea that agonist-receptor interaction (vasopressin, in this case) initiates [Ca 2+] i-waves that then propagate via some form of Ca 2+-induced release of Ca 2+, perhaps in a manner similar to that proposed by Berridge and Irvine [1].