Abstract
1. 1. Crude synaptosomal fractions obtained from four areas of rat brain were studied: cerebral cortex, hippocampus, midbrain, thalamus with hypothalamus, using the Cotman & Matthews method (1971) Biochim. biophys. Acta 249, 350–394. 2. 2. The purity of synaptosomal fractions was controlled by electron microscopy, and by determination of some marker enzymes such as: LDH, MAO, AChE and cytochrome-c oxidase. 3. 3. Synaptosomes were disrupted by hypoosmotical shock. 4. 4. Crude synaptosomal membrane preparations indicated on increased Ca 2+Mg 2+-ATPase activity in comparison to the activity of this enzyme in synaptosomal fractions. 5. 5. The incubation of crude synaptosomal membranes with cAMP and theophylline caused the subsequent increase of Ca 2+Mg 2+-ATPase activity, but mainly, in hippocampal region.
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