Abstract
The effects of a Ca 2+-ATPase inhibitor, cyclopiazonic acid (CPA), and two hydroquinone-antioxidants, 2,5-di-( tert-butyl)-1,4-hydroquinone (DTBHQ) and 2,5-di-( tert-amyl)-1,4-hydroquinone (DTAHQ) on the release of IL-4 and MCP-1 from RBL-2H3 cells were investigated. CPA, DTBHQ and DTAHQ, all of which induce intracellular free Ca 2+ concentration ([Ca 2+] i) increase, induced IL-4 and MCP-1 release in a dose-dependent manner. The release of TNF- α required both a Ca 2+-ATPase inhibitor and 12- O-tetradecanoylphorbol-13-acetate (TPA). However, the Ca 2+-ATPase inhibitors induced IL-4 and MCP-1 production without TPA. The release of IL-4 and MCP-1 reached a maximum at 9 and 6 h, respectively. IL-4 and MCP-1 release was inhibited by treatment with the immunosuppressant FK-506 and actinomycin D. Therefore, in our system IL-4 and MCP-1 release involves Ca 2+-dependent and FK-506-sensitive signaling pathways. This is the first report about Th-2 type cytokine and chemokine production in RBL-2H3 cells.
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