Abstract
ROS-GC1 belongs to the Ca2+-modulated sub-family of membrane guanylate cyclases. It primarily exists and is linked with signaling of the sensory neurons – sight, smell, taste, and pinealocytes. Exceptionally, it is also present and is Ca2+-modulated in t he non-neuronal cells, the sperm cells in the testes, where S100B protein serves as its Ca2+ sensor. The present report demonstrates the identification of an additional Ca2+ sensor of ROS-GC1 in the testes, neurocalcin δ. Through mouse molecular genetic models, it compares and quantifies the relative input of the S100B and neurocalcin δ in regulating the Ca2+ signaling of ROS-GC1 transduction machinery, and via immunochemistry it demonstrates the co-presence of neurocalcin δ and ROS-GC1 in the spermatogenic cells of the testes. The suggestion is that in more ways than one the Ca2+-modulated ROS-GC1 transduction system is linked with the testicular function. This non-neuronal transduction system may represent an illustration of the ROS-GC1 expanding role in the trans-signaling of the neural and non-neural systems.
Highlights
Rod outer segment membrane guanylate cyclase, ROS-GC1, belongs to the family of membrane guanylate cyclases
It is co-expressed with calcium sensor S100B and neuronal calcium sensor proteins GCAP1 and Neurocalcin δ (NCδ) (Jankowska et al, 2008)
Using total RNA isolated from these tissues individual cDNA libraries were constructed and used for amplification of specific fragments of ROS-GC1 and NCδ cDNAs by polymerase chain reaction
Summary
Rod outer segment membrane guanylate cyclase, ROS-GC1 (known as Ret-GC1 or GC-E), belongs to the family of membrane guanylate cyclases. Its discovery and molecular characterization was a land mark event in the field of phototransduction (reviewed in Pugh et al, 1997; Koch et al, 2010) as it identified the source of cyclic GMP that serves as a second messenger of the LIGHT signal. It impacted the entire membrane guanylate cyclase field by dividing the guanylate cyclase family into two subfamilies, one comprising the hormone receptor cyclases, and the other, cyclases modulated by intracellular [Ca2+]i signals (reviewed in: Sharma, 2010). Ca2+ signaling of ROS-GC1 activity mediated by neurocalcin δ is operative in retinal ganglion cells (Krishnan et al, 2004); and of ONE-GC in the olfactory neuroepithelium (Duda et al, 2001a, 2004)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
