Abstract
The stability of unilamellar vesicles during freeze-thaw cycles strongly depends on the Ca 2+ concentration in the aqueous solution. Experiments performed at equal ionic strengths with Na + and Ca 2+ solutions indicate that the effect observed is specific for Ca 2+. This is interpreted to be a consequence of the adsorption of Ca 2+ on the vesicle bilayers. The variation of lipid and Ca 2+ concentrations indicates that stability is achieved at a particular Ca 2+/lipid ratio of 8 mol/mol above which vesicles are stable. The stability appears to be mainly conferred by the external Ca 2+ in both slow and rapid cycles, independent of the ionic vesicle content. However, internal Ca 2+ seems to increase the stability according to the F T cycle rate to some extent in the absence of Ca 2+ in the external Solution.
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