C677T genotypes in methyltetrahydrofolate reductase gene in student obesity

Abstract

Obesity is known as weight gaining programme which leads to increase in body mass index (BMI), is known for significant risk factors for chronic diseases. The prevalence of obesity has been rising in South Asian countries, specifically in Pakistan. Numerous studies have shown the association with metabolic diseases (ex; obesity) with single nucleotide polymorphisms. The present study has been designed to investigate the association between C677T polymorphism in methylenetetrahydrofolate reductase (MTHFR) gene with obesity in Pakistan population. In this case-control study, we have enrolled 200 obese students and 200 non-obese or control students based on the BMI. Genomic DNA was isolated with enrolled 400 blood samples and PCR was performed with C677T polymorphism in the MTHFR gene to analyse the frequency. Later on restriction enzyme length polymorphism analysis was carried out for the undigested PCR products. Genotyping frequencies for C677T polymorphism were compared between obesity and non-obesity students. Genotype, allele and various genetic model of inheritances were calculated and documented the significant association (p < 0.05). However, Anova analysis couldn’t document the positive association when we measured with C677T polymorphism in obesity cases versus anthropometric measurement such as age, weight and BMI (p > 0.05). The present study concludes C677T polymorphism in MTHFR gene was genetically associated between the obesity risks in Pakistan population. However, relation with Anova analysis showed the negative association with C677T polymorphism.