C60 FULLERENE EFFECT ON THE DYNAMICS OF FATIGUE PROCESSES IN RAT SOLEUS MUSCLE AFTER ISCHEMIA-REPERFUSION

Abstract

43 The purposeful application of bio compatible nanomaterials of low toxicity is a relevant problem in modern biotechnology. It is assumed that the use of nanoparticles will help to solve the problems of early disease diagnostics and targeted delivery of drugs into tissues and cells, as well as new methods of selective therapies. C60 fullerene has a prominent position among other potentially effective therapeutic agents [1]. It is known that C60 molecule normalizes cellular metabolism and neural processes, increases resistance to stress, exhibits antiviral properties, has pronounced anti-inflammatory and anti-allergic effects, enhances activity of enzymes and regenerative capacity of tissues [1]. Finally, C60 fullerene and its derivatives can be used for the treatment of cancer [2–4]. C60 fullerene can be readily dissolved in organic solvents, yet it is practically insoluble in water [5]. Poor solubility in water severely hampers investigation of physiological and pharmacological effects of C60 fullerene. As a consequence, a number of C60 fullerene derivatives with better solubility in polar solvents have been synthesized to date [5–6]. Pristine C60 fullerene aqueous solution (С60FAS) has been received by transferring C60 molecules from toluene to water followed by sonication [7–8]. The dark brown solution was identified to be a typical colloid system containing a single C60 molecule and spherical clusters with the diameter of 2–3 nm or more (depending on the C60 fullerene concentration in water) in a hydrated state [7–9]. Due to its nanoscale dimensions, the watersoluble pristine C60 fullerene can penetrate the cell membrane [10]. Toxicity is a major concern for the use of C60 fullerene-containing drugs for biomedical УДК 546.26.043