Abstract
Understanding the structure of 30-nm chromatin fiber, typically regarded as the secondary structure of DNA, is one of the keys to illuminating the molecular mechanism of transcription and epigenetic regulation. How nucleosome arrays fold into chromatin fibers has been remained elusive for over three decades. Cryo-electron microscopy (cryo-EM) provides a suitable tool for solving this puzzle. We have recently solved the 3-D cryo-EM structure of the 30-nm chromatin fiber reconstituted in vitro in the presence of linker histone H1 at resolution of about 11 Ä, which shows a histone H1-dependent left-handed double-helical architecture with twist of repeating tetra-nucleosomal structural units (Fig. 1 ) [ 1 ]. This result and some further EM studies on chromatin fibers will be presented. ...
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