C1QBP suppresses cell adhesion and metastasis of renal carcinoma cells

Yong Wang,Yin Sun,Jing Su,Dan Yue,Yajing Chen,Yuanjie Niu,Can Qi,Donghe Fu,Ning Zhang

doi:10.1038/s41598-017-01084-w

Abstract

Complement component 1q subcomponent binding protein (C1QBP) is a ubiquitously expressed cellular protein and can be upregulated or activated in a variety of malignant tumors, including those from thyroid, colon and breast, but its role remains unclear in renal cell carcinoma (RCC). In this study, C1QBP knockdown in RCC cell influenced expression of multiple genes associated with cell adhesion, among which L1 cell adhesion molecule (L1CAM) was significantly higher upon a reduction of C1QBP. In turn, cell adhesion and invasion abilities were significantly increased with increased metastasis to lung and liver in vivo. C1QBP may regulate RCC cell adhesion and invasion through influencing the p-GSK3/β-Catenin/L1CAM expression. Over all, our study demonstrated that C1QBP could regulate RCC metastasis by regulating the GSK3/β-Catenin/L1CAM signaling pathway.

Highlights

Complement component 1q subcomponent binding protein (C1QBP) known as HABP1, p32 and gC1qR, is a ubiquitously expressed, multi-ligand-binding, multicompartmental cellular protein involved in various ligand-mediated cellular responses[1]
C1QBP is found to be essential for cancer cell chemotaxis and metastasis, its expression level is closely linked with distant metastasis and TNM stages in breast cancer[8], and there is a positive correlation between the expression of C1QBP and Gleason score, pathologic stage, tumor recurrence in prostate cancer patients
It has been documented that C1QBP is significantly decreased in human cervical squamous cell carcinoma tissues relative to normal cervix tissues and inhibits viability, migration and proliferation of cervical squamous cells carcinoma via the p38 MAPK signaling pathway[9]

Summary

Introduction

Complement component 1q subcomponent binding protein (C1QBP) known as HABP1, p32 and gC1qR, is a ubiquitously expressed, multi-ligand-binding, multicompartmental cellular protein involved in various ligand-mediated cellular responses[1] It is widely present on mitochondria, nucleus, cytoplasm, Golgi apparatus and cell membranes, and can be secreted into extracellular matrix. Consistent with the finding in cervical squamous cell carcinoma, our study[10] shows that the level of C1QBP in the renal carcinoma tissues is significantly lower than that in the adjacent normal tissues, and the expression of C1QBP could be used as an independent prognostic maker for cancer progression in the renal cell carcinoma (RCC) patients. Studies investigating the role of C1QBP in spontaneous metastasis of RCC cells with a focus on RCC adhesion and metastasis

Methods

Results

Conclusion