Abstract
C-terminus kinesin motor KIFC1 is known for centrosome clustering in cancer cells with supernumerary centrosomes. KIFC1 crosslinks and glides on microtubules (MT) to assist normal bipolar spindle formation to avoid multi-polar cell division, which might be fatal. Testis cancer is the most common human cancer among young men. However, the gene expression profiles of testis cancer is still not complete and the expression of the C-terminus kinesin motor KIFC1 in testis cancer has not yet been examined. We found that KIFC1 is enriched in seminoma tissues in both mRNA level and protein level, and is specifically enriched in the cells that divide actively. Cell experiments showed that KIFC1 may be essential in cell division, but not essential in metastasis. Based on subcellular immuno-florescent staining results, we also described the localization of KIFC1 during cell cycle. By expressing ΔC-FLAG peptide in the cells, we found that the tail domain of KIFC1 might be essential for the dynamic disassociation of KIFC1, and the motor domain of KIFC1 might be essential for the degradation of KIFC1. Our work provides a new perspective for seminoma research.
Highlights
Testis cancer is the most common cancer type among young men, and seminoma is one of the most common testis cancers in the world
By expressing ΔC-FLAG peptide in the cells, we found that the tail domain of KIFC1 might be essential for the dynamic disassociation of KIFC1, and the motor domain of KIFC1 might be essential for the degradation of KIFC1
The first thing we did was to evaluate and compare the KIFC1 expression level in muscle, testis and seminoma tissue to determine whether KIFC1 is important in testis cancer development
Summary
Testis cancer is the most common cancer type among young men, and seminoma is one of the most common testis cancers in the world. It has been reported that human KIFC3 is highly expressed in human docetaxel resistant breast cancers, and increases the amount of free tubulins in human breast cancer. KIFC1 is found to be enriched in numerous of cancer types [8,9,10,11,12,13,14], but there is no report on testis cancers. Other functions of KIFC1 in cancer cells including driving tumor malignancy by interacting with www.impactjournals.com/oncotarget cyclins [20] and inducing drug resistance against taxane [21] and tamoxifane [22] in breast [9] and/or prostate cancer [21]. It is found to be essential in vesicular and organelle trafficking [24,25,26,27], spermiogenesis [28], oocyte development [29] and embryo gestation [30]
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