Abstract

C-phycocyanin (PC) from a red alga, Porphyra tenera was separated into α and β subunits by SDS-polyacrylamide gel electrophoresis and Bio-Rex 70 column chromatography. The absorption and fluorescence maxima of the phycocyanin were 615 and 632 nm, respectively. The amino acid compositions of phycocyanin and its subunits were predominantly acidic amino acids. The molecular weights of phycocyanin and its subunits were determined to be 111 000 (PC), 18 100 (α) and 22 200 (β) by the sedimentation equilibrium method. It was assumed that the phycocyanin forms a trimeric aggregate, the trimer (αβ) 3 at pH 7.0.

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