Abstract
The biosynthetic origin of methyl groups in heme d1 isolated from the nitrite reductase cytochrome cd1 was investigated by a stable isotope labeling experiment. Pseudomonas aeruginosa (American Type Culture Collection strain 19429) was grown on a minimal medium supplemented with [13C]methionine. The enzyme was purified, the heme extracted, converted into the free base methyl ester derivative, and purified. 1H NMR and 13C NMR indicated that only the methyl groups attached to C2 and C7 are derived from methionine.
Highlights
The biosynthetic origin of methyl groups in heme d, isolated from the nitrite reductase cytochrome cdl was investigated by a stable isotope labeling experiment
NMR and 13C NMR indicated that only the methyl groups attached to C2 and C7 are derived from methionine
For these structures it has been shown (Scheme I) that the methyl group is added to the pyrrolic carbon by C-methylation, in which S-adenosylmethionine (SAM) is the methyl donor and uroporphyrinogen III is the acceptor (3)
Summary
C. Baker, The biosynthetic origin of methyl groups in heme d, isolated from the nitrite reductase cytochrome cdl was investigated by a stable isotope labeling experiment. The enzyme was purified, the heme extracted, converted into the free base methyl ester derivative, and purified. NMR and 13C NMR indicated that only the methyl groups attached to C2 and C7 are derived from methionine.
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