Abstract
The transportation of water and cryoprotective agents across the cell membrane plays an important role in cell survival during freezing and thawing of cells. A microperfusion chamber was developed for studying cellular response to osmotic change. The cells are immobilized in the microperfusion chamber and exposed to the solution which is osmotic solution shift to hypertonic solution. The change of extracellular concentration has a certain impact on fitting cell membrane permeability parameters. The microperfusion chamber can be modeled through the mechanism of convection diffusion, and thus the Finite Element Analysis (FEA) was applied for the simulation of extracellular concentration. The validity of the concentration shift model was confirmed by comparing the cell-free experimental results used by the optical image processing with the results of simulation model. The CuCl 2 solution was used to do the cell-free experiment and NaCl solution was used to do the determined cell membrane permeabilities experiment, thus the dynamic viscosity and diffusion coefficient of the solution at the certain concentration difference were measured to ensure the accuracy of the stimulant results. The simulation results have shown that the T-pipe and Y-pipe make no difference on the concentration change, but the size of T-pipe has a significant impact on extracellular NaCl concentration. The equilibration time of the low concentration shift to high concentration is about 100s, and the simulation shows the equilibration time of concentration function affects the measurement of hydraulic conductivity. Thus using FEA to analysis the extracellular concentration profile of microperfusion chamber has a great prospect to service for the cryobiology research by improving the precision of the experimentally determined cell membrane permeabilities.
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