Bxy-fuca encoding α-L-fucosidase plays crucial roles in development and reproduction of the pathogenic pinewood nematode, Bursaphelenchus xylophilus.

Abstract

The pine wood nematode (PWN) Bursaphelenchus xylophilus is the causal agent of pine wilt disease (PWD). This disease is a serious threat to pine forests globally. The fuca gene encodes α-L-fucosidase, which plays crucial roles in numerous biological and pathological processes in bacteria, fungi, plants and animals. To find promising control strategies against PWD, we investigated the expression and functions of Bxy-fuca in B. xylophilus. Bxy-fuca encoding α-L-fucosidase is highly conserved within the deduced functional domains and key residues. It is expressed continuously across all developmental stages of B. xylophilus. mRNA in situ hybridization demonstrated that Bxy-fuca was mainly localized in the body wall muscles and intestine. RNA interference indicated that the zygotic expression of Bxy-fuca was indispensable for embryogenesis. The rate of B. xylophilus egg hatch was significantly decreased after Bxy-fuca was interfered. Postembryonic silence of Bxy-fuca resulted in a dramatic decrease in the longevity of and the total number of eggs produced by B. xylophilus. In addition, the motility of the nematode was greatly hampered with a significant drop in head thrashing frequency. Bxy-fuca plays crucial roles in development, lifespan and reproduction of B. xylophilus. Our results provide promising hints for control of PWD by blocking embryogenesis and ontogenesis, decreasing nematode fecundity, and disrupting the connection between B. xylophilus and its vector beetle by preventing nematode movement into the tracheal system. © 2019 Society of Chemical Industry.