Butterbur (Petasites hybridus) Extract Ameliorates Hepatic Damage Induced by Ovalbumin in Mice.

Rana M Alhusayan,Saad Alkahtani,Khalid Elfakki Ibrahim,Doaa M El-Nagar,Badr Abdullah Aldahmash,Ahmad Rady,Madhukar Saxena

doi:10.1155/2020/3178214

Rana M Alhusayan, Saad Alkahtani + Show 5 more

Open Access

https://doi.org/10.1155/2020/3178214

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Abstract

The liver is the most vital organ that could be influenced by inducers of hypersensitivity such as ovalbumin. The current study was carried out to explore the effects of butterbur (Petasites hybridus) extract on the ovalbumin-induced liver hypersensitivity in Swiss albino male mice. Animals were divided into 4 groups, 1st group served as a control group, 2nd group treated with daily oral administration of 75 mg/kg of butterbur extract, 3rd group received single oral dose 100 mg/kg of ovalbumin to induce hypersensitivity, and 4th group treated with oral administration of butterbur extract one-day post to the hypersensitivity induction. Ovalbumin induces a significant increase in the activity of liver enzymes and MDA and decreased the activity of CAT after the ovalbumin treatment. Histopathological investigations revealed marked pathological alterations in liver tissues in the form of hyaline degeneration and fibrosis. Additionally, heavy immune response indicated by immunostaining of MDA and TNF-α could be observed. In contrast, posttreatment with butterbur extract after hypersensitivity induction resulted in a significant decrease of liver enzymes and oxidative stress and reduced the inflammation and fibrosis of liver tissues. These results suggest that butterbur extract is considered as anti-inflammatory and antioxidant therapeutic herb for hypersensitivity treatment of liver.

Highlights

The liver is the largest organ in the mammalian body that plays a main role in proteins, lipids, and carbohydrate metabolism [1]
Ovalbumin is known to induce asthma by causing chronic airway inflammation manifested by infiltration of neutrophils, eosinophils, and lymphocytes into bronchial lumen with increasing of reactive nitrogen and reactive oxygen species (ROS) levels
Animals were randomly divided into 4 groups, 1st group served as the control group, 2nd group treated with daily oral administration of 75 mg/kg of butterbur extract [18], 3rd group received single oral dose 100 mg/kg of ovalbumin to induce hypersensitivity [19], and 4th group treated with oral administration of butterbur extract one-day post to the hypersensitivity induction

Summary

Introduction

The liver is the largest organ in the mammalian body that plays a main role in proteins, lipids, and carbohydrate metabolism [1]. Inflammation and ROS production might induce liver injury, as well as lung damage ([6, 7]; Abdsmall et al, 2018). Herbal remedies attract growing interest as an alternative of chemical therapy, because of their potential role in reducing pathological changes and oxidative stress ([8,9,10]; ; [11]). The main active constituents of butterbur are petasin and isopetasin [15]. Both are responsible for the inhibition of leukotriene synthesis and inflammation. We illustrate the potential effect of butterbur extract to reduce the ovalbumin-induced inflammation and liver damages in albino mice

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