Buteinylated-hafnium oxide bionanoparticles for electrochemical sensing of wogonin.

Abstract

Hybridizing biomolecules with metal oxide nanostructures possessing inherent optical emission and electrochemical functionality is advantageous for external mediator-free analytical applications. This work demonstrates the ultrasonochemical synthesis of hafnium oxide (HfO2) nanoparticles and their combination with butein, a chalcone type polyphenol, for the direct electrochemical detection of active herbaceuticals. The underlying hybridization chemistry between HfO2 and butein within the bio-nano interface is comprehensively investigated using ultraviolet diffuse reflectance, X-ray diffraction, Fourier-transform infrared, and X-ray photoelectron spectroscopic techniques. Electron micrographs suggest the formation of elongated nano spherical particles of HfO2 with the incorporation of butein (average particle size of 17.6 ± 2.9 nm). The catecholic OH group of butein existing on the surface of hybridized HfO2 exhibits reversible redox behavior convenient for probing the selected target analyte at physiological pH. The electron diffusion kinetics, electron transfer coefficient and rate constant parameters of the prepared HfO2-butein electrode material have been studied in detail for further application in biomolecular sensing of wogonin. The as-developed sensor platform exhibits a linear detection range of 20-100 μM with a current density of 60 μA cm-2 and a detection limit of 0.63 μM, which is promising for herbaceutical analysis.