Butanol extract of Morinda Lucida (BEML) protects against Isoproterenol‐induced myocardial infarction in Wistar rats

Abstract

With the rising rates of cardiovascular diseases, increase cost of modern therapeutic drugs, as well as the adverse effects of the modern medicine on the users; there is a surge in the need to search for herbs with excellent antioxidant properties for use in preventive medicine. Morinda Lucida, commonly found in the Tropics, precisely in the rain forest of West Africa; it is an important medicinal plant known for its antioxidant and therapeutic uses. The aim of this study was to investigate the cardioprotective effects of the Butanol extract of Morinda Lucida (BEML) against Isoproterenol‐induced myocardial infarction in Wistar rats. Male albino Wistar rats (n=60) were pre‐treated with BEML for a period of 10 days. Group A (control) received food and water ad‐libitum. Group B was challenged with 70 mg/kg Isoproterenol (ISO) alone. Group C: Isoproterenol + 100 mg/kg BEML. Group D: Isoproterenol + 200 mg/kg BEML Group E: 100 mg/kg BEML alone and Group F: 200 mg/kg BEML alone. Groups B, C and D were challenged with ISO on the 11th day. This study found out that ISO induced severe myocardial injuries associated with significant increase in markers of oxidative stress, as confirmed by elevated Malondialdehyde (MDA), as well as decreased reduced Glutathione (GSH), Glutathione peroxidase (GPx), Glutathione, Glutathione‐S‐transferase (GST), Catalase (CAT), Superoxide dismutase (SOD) relative to ISO alone. Pre‐treatment with BEML prior to administration of ISO significantly reduced serum Creatinine kinase myocardial band (CK‐MB) and Lactate dehydrogenase (LDH and also suppressed the expressions of Cardiac troponin I (CTnI), and nuclear factor kappa beta (NF‐κB), respectively. Pre‐treatment with BEML reduced infarct size and restored cardiac ultrastructural integrity. Gas Chromatography Mass Spectrometry (GCMS) analysis revealed the presence of Phytol, 11‐Eicosenoic acid, Palmitoleic acid, Nonacosylic acid, Methyl Palmitoleate, Stearic acid, Vitamin E (alpha‐Tocopherol), Tocopheryl acetate, 17α‐Hydroxyprogesterone, β‐Amyrin acetate, Alpha‐Amyrin acetate, lupenone, and Sclareol in BEML. Together, we conclude that BEML act as potential protective agent against myocardial infarction and other cardiac conditions through its antioxidant, anti‐inflammatory and free radical scavenging activity. The observed cardio‐protective effect might be due to the presence of arrays of phytonutrients.Support or Funding InformationNoneThis abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.