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Abstract
By understanding the hormonal action behind the induction of spermiation in Leptodactylus ocellatus can help in the collection of semen for artificial insemination and cryopreservation. The experiments were conducted at the Experimental Frog Farm of the Federal University of Vicosa. Seven L. ocellatus males with the following secondary sexual characteristics were selected: black thorns, developed forelimbs, and amplexus reflex. Spermiation was induced in these animals by applying daily 0.4 μg buserelin acetate hormone (GnRHa) for 7 days, being the semen collected 90 min after the application. The volume, color, vigor, motility, sperm concentration, and spermatozoa morphology were subsequently evaluated. Three animals responded to GnRHa induction, enabling the collection of seven samples of semen, and the first sample was collected 97.5h after the first application. The semen of the creole frog presented the following characteristics: an average volume of 0.38 mL, murky color, sperm vigor of 3.71, sperm motility of 77.14%, sperm concentration of 6.60 x 10 6 SPTZ mL -1 , and 69% of normal sperm. GnRHa can induce spermiation in the creole frog. Although the volume of collected semen was low, the color, vigor, motility, concentration, and spermatozoa content showed to be adequate.
Highlights
Degradation of natural ecosystems by human activities (YOUNG et al, 2000), introduction of exotic species such as the bullfrog, and hunting are reasons for the decline in amphibian population in different parts of the world (JUNCÁ, 2001)
This study developed a hormonal induction technique for L. ocellatus by applying the methodology used in bullfrogs
Thirty-four L. ocellatus specimens were collected from a natural environment over a period of 34 days
Summary
Degradation of natural ecosystems by human activities (YOUNG et al, 2000), introduction of exotic species such as the bullfrog, and hunting are reasons for the decline in amphibian population in different parts of the world (JUNCÁ, 2001). The rearing of native species like Leptodactylus ocellatus for return to the wild can help in lessening this problem. Understanding the process of reproduction and the. Biological Sciences action of reproductive hormones is important when using induction-based methods to obtain gametes for cryopreservation and artificial fertilization. Fertilization is an important tool in aquaculture that facilitates the storage and transport of gametes (GOLDBERG et al, 2002). Due to the lack of studies on L. ocellatus biology, especially on its reproductive physiology and gametes, it becomes difficult to study this organism; which reinforces the need for further research on this Brazilian species
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