Burn wound healing effects of the root hydroethanolic extract of Cochlospermum planchonii in mice

Abstract

Cochlospermum planchonii is a plant traditionally used in Togo to treat burn wounds. The present study aimed at evaluating the effect of the root hydroethanolic extract of Cochlospermum planchonii on the skin burn wounds. Burns were induced in ICR mice by applying on the skin of the dorsal region with a metal stick (0.7 cm diameter) preheated at 100 °C for 10 seconds. Four (4) groups of 8 mice were formed, Control Group (I); Group II, III and IV treated respectively with C. planchonii gel 2.5%, 5%, and Brulex® (Zinc oxide 15% cream). The surfaces of the wounds were evaluated daily by the ImageJ software. Biopsies were performed on day six and twelve for hydroxyproline assays and histological examinations. On the 12th day, wound contraction rates were 44.12%, 67.82%, 66.68% and 69.32% respectively for Group I, Group II, Group III and Group IV. Hydroxyproline levels on day 12 were significantly higher in samples of animals treated with C. planchonii 2.5% and 5% (+114,93 ± 1,61 and +120,54 ± 7,22 of augmentation respectively, P<0.01 when compared to the control group). Histological examination confirmed these observations. C. planchonii root hydroethanolic extract could be a potentially natural remedy for burn wound.