Bumble Bee Foraged Pollen Analyses in Spring Time in Southern Estonia Shows Abundant Food Sources.

Abstract

Simple SummaryPollinators make a strong contribution to ecosystem stability. However, nowadays, they also need protection and sustainable habitat to live and develop. Not all regions can provide suitable habitats due to agricultural intensification, urbanization, climate changes and corresponding impacts. Our study was conducted in the late spring in south Estonia where arable lands were surrounded by forest patches and rural areas. For better performance, we used both light microscopy and DNA metabarcoding methods for pollen identification. We found that bumble bees foraged on the diverse food sources showing preferences for several main plant families. Additionally, in our case, land-use types did not show important effects on bumble bee food choices and foraging decisions. Various landscape features can provide diverse food sources at the early development stages and support nest longevity. Here, we can say that a better understanding of pollinators’ food preferences can help in the application of more suitable measures for their conservation. Agricultural landscapes usually provide higher quantities of single-source food, which are noticeably lacking in diversity and might thus have low nutrient value for bumble bee colony development. Here, in this study, we analysed the pollen foraging preferences over a large territory of a heterogeneous agricultural landscape: southern Estonia. We aimed to assess the botanical diversity of bumble bee food plants in the spring time there. We looked for preferences for some food plants or signs of food shortage that could be associated with any particular landscape features. For this purpose, we took Bombus terrestris commercial hives to the landscape, performed microscopy analyses and improved the results with the innovative DNA metabarcoding technique to determine the botanical origin of bumble bee-collected pollen. We found high variability of forage plants with no strong relationship with any particular landscape features. Based on the low number of plant species in single flights, we deduce that the availability of main forage plants is sufficient indicating rich forage availabilities. Despite specific limitations, we saw strong correlations between microscopy and DNA metabarcoding data usable for quantification analyses. As a conclusion, we saw that the spring-time vegetation in southern Estonia can support bumble bee colony development regardless of the detailed landscape structure. The absence of clearly dominating food preference by the tested generalist bumble bee species B. terrestris makes us suggest that other bumble bee species, at least food generalists, should also find plenty of forage in their early development phase.