Bull�s-eyes immunological synapses(ISs) ,but not multifocal ISs are formed between dendritic cells and T cells

Abstract

Event Abstract Back to Event Bull’s-eyes immunological synapses(ISs) ,but not multifocal ISs are formed between dendritic cells and T cells Wei Lin1, Zhichao Fan2, 3, 4, Xunbin Wei2, 3, 4 and Yiwei Chu1* 1 Department of Immunology, Shanghai Medical College, Biotherapy Research Center of Fudan University,Shanghai, China 2 Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China 3 Department of Chemistry, Fudan University, Shanghai, China 4 Institutes of Biomedical Sciences, Fudan University, Shanghai, China Immunological synapses (ISs) are multimolecular assembly place of receptors and adhesion molecules, formed at the interface of T cells and antigen presenting cells (APCs) during antigen recognition. They play a central role in T cells activation. Usually, multifocal ISs are formed between dendritic cell (DC) and naive CD4+T cells or CD8+T cells as well as activated CD4+T cells. On the contrary, classical bull's-eye ISs are formed between B cells, supported planar bilayers and naïve or activated T cells. In our experiments, we found interestingly that bull's-eye ISs, but not multifocal ISs are formed at the murine DC-T cells interface induced by staphylococcal Enterotoxin B (SEB) stimulation. Similar to the bull's-eye ISs of B-T cells, bull's-eye ISs of DC-T cells have mature classical shape and size, and lead to the long time stable contact between DC and T cells. Compared with the multifocal synapses, bull's-eye ISs lead to different calcium flux in T cells. A dynamic analysis revealed that the mature DCs form more bull's-eye ISs and long-lasting interactions conjugates with naive T cells than immature DCs. Further analysis of the molecular mechanism of bull's-eyes ISs, we found some costimulatory molecules, such as ICAM-1, acting as an important molecular associated with cell cytoskeletal, dynamically participate in formation of bull's-eye ISs. Our results provide an alternative synapse formation between DC-T cells, which will potentially explore the new signal pathway in T cells activation. Acknowledgements We would like to acknowledge funding by Key Project of Chinese National Programs for Fundamental Research and Development (973 program 2011CB910404). And we acknowledge Professor Kenneth L. Rock of UMass Medical School, Professor Matthew F. Krummel of University of California, San Francisco and Professor Francis R. Carbone of University of Melbourne. They gave us the DC2.4 cell line and plasimid of OT-I/II TCR and sequences of them as gifts, separately. Keywords: T cells, Dendritic Cells, staphylococcal Enterotoxin B, Bull's-eyes immunological synapse;, living cells imaging Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune receptors and signaling Citation: Lin W, Fan Z, Wei X and Chu Y (2013). Bull’s-eyes immunological synapses(ISs) ,but not multifocal ISs are formed between dendritic cells and T cells. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00856 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 22 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Yiwei Chu, Department of Immunology, Shanghai Medical College, Biotherapy Research Center of Fudan University,Shanghai, Shanghai, 200032, China, ywchu@shmu.edu.cn Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Wei Lin Zhichao Fan Xunbin Wei Yiwei Chu Google Wei Lin Zhichao Fan Xunbin Wei Yiwei Chu Google Scholar Wei Lin Zhichao Fan Xunbin Wei Yiwei Chu PubMed Wei Lin Zhichao Fan Xunbin Wei Yiwei Chu Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.