Bulk automated analysis of variability of nucleosome structures and their complexes

Abstract

The cryo-EM revolution is now driving the progress in our understanding of chromatin structure by solving the structures of nucleosomes and their complexes. The number of structures in the PDB database has doubled over the last three years and is rapidly growing. However, harnessing helpful information from this wealth of structural 3D data is challenging. Variations in components’ naming nomenclature, chain identifiers, amino acids, and nucleotide numbering and lack of readily available tools for systematic structure analysis and comparison adds to the complexity of the problem.