Abstract
Protocol for bulblet regeneration from tepal segments was developed in Asiatic hybrid lily cultivars. The explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of ∞-naphthalene acetic acid (NAA) and 6-benzyladenine (BA). The days for bulblet initiation decreased with the addition of NAA and/or BA in the medium. The bulblets were initiated much earlier (26.9 d) with 1 mg l−1 NAA and 0.5 mg l−1 BA. About 75% of the cultures with 1.2 bulblets per explants were observed in growth regulator-free medium. However, the regeneration response increased to 80.82% with 1.5 mg l−1 NAA and 0.5 mg l−l BA. The number of bulblets per explant and average fresh weight was highest with 1.5 and 1 mg l−1 NAA in combination with 0.5 mg l−1 BA. Among the cultivars, maximum number of bulblets was recorded for ‘Alaska’, whereas heaviest bulblets were observed in ‘Pollyana’. The bulblets attaining the size of 14–16 cm flowered in two and a half years without any phenotypic variations. ...
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
