Bulbectomy of neonatal mice induces migration of basal cells from the olfactory epithelium

Abstract

Bulbectomy of neonatal mice induced cell migration from the olfactory epithelium in the nasal septum. We examined cell types of migrating clusters by immunohistochemistry using anti-keratin and anti-BrdU antibodies, and by electron microscopy. At 1–2 days after unilateral bulbectomy of P1 mice, cells migrated from the olfactory epithelium to the lamina propria of the septal olfactory mucosa. Horizontal basal cells that reacted specifically with anti-keratin antibody, and globose basal cells characterized by a round shape and poor content of organellae in their cytoplasm, were contained in the cluster. At 1 week, migrated clusters that contained keratin-positive horizontal basal cells were observed in both the lamina propria and olfactory bulb on the unoperated side. At 1 month, not only basal cells but also olfactory cells and presumed supporting cells were involved in the clusters in the lamina propria and olfactory bulb, suggesting that migrated cells do not transform to other phenotypes.