Abstract
Over the past 30 years, we have performed many fundamental studies on two Oryza sativa subsp. indica varieties, Zhenshan 97 (ZS97) and Minghui 63 (MH63). To improve the resolution of many of these investigations, we generated two reference-quality reference genome assemblies using the most advanced sequencing technologies. Using PacBio SMRT technology, we produced over 108 (ZS97) and 174 (MH63) Gb of raw sequence data from 166 (ZS97) and 209 (MH63) pools of BAC clones, and generated ~97 (ZS97) and ~74 (MH63) Gb of paired-end whole-genome shotgun (WGS) sequence data with Illumina sequencing technology. With these data, we successfully assembled two platinum standard reference genomes that have been publicly released. Here we provide the full sets of raw data used to generate these two reference genome assemblies. These data sets can be used to test new programs for better genome assembly and annotation, aid in the discovery of new insights into genome structure, function, and evolution, and help to provide essential support to biological research in general.
Highlights
Background & SummaryRice is the leading staple crop for mankind and has been recognized as an important model organism for biological research, especially for monocot plants
The Zhenshan 97 (ZS97), Minghui 63 (MH63), Shanyou 63 (SY63) hybrid system has been used as a model[4,5,6,7,8,9] over the past 30 years, and concomitantly our lab has made a series of attempts to gain a fundamental understanding of the genetic basis of heterosis—a biological mystery that has puzzled the scientific community for more than 100 years
We initiated a joint collaborative project to generate two reference-quality genome assemblies for ZS97 and MH63 to be used as a fundamental tool to help us understand the underlying molecular genetic basis of heterosis[10]
Summary
Rice is the leading staple crop for mankind and has been recognized as an important model organism for biological research, especially for monocot plants. We initiated a joint collaborative project to generate two reference-quality genome assemblies for ZS97 and MH63 to be used as a fundamental tool to help us understand the underlying molecular genetic basis of heterosis[10]. With the resources and data generated in this study, we were able to de novo assemble two reference-quality genome sequences, but we were able to provide the scientific community with data to advance biological research at the genomic level, especially for further understanding of the genetic basis of heterosis
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