Abstract
BackgroundCurrently available microscope slide scanners produce whole slide images at various resolutions from histological sections. Nevertheless, acquisition area and so visualization of large tissue samples are limited by the standardized size of glass slides, used daily in pathology departments. The proposed solution has been developed to build composite virtual slides from images of large tumor fragments.Materials and methodsImages of HES or immunostained histological sections of carefully labeled fragments from a representative slice of breast carcinoma were acquired with a digital slide scanner at a magnification of 20×. The tiling program involves three steps: the straightening of tissue fragment images using polynomial interpolation method, and the building and assembling of strips of contiguous tissue sample whole slide images in × and y directions. The final image is saved in a pyramidal BigTiff file format. The program has been tested on several tumor slices. A correlation quality control has been done on five images artificially cut.ResultsSixty tumor slices from twenty surgical specimens, cut into two to twenty six pieces, were reconstructed. A median of 98.71% is obtained by computing the correlation coefficients between native and reconstructed images for quality control.ConclusionsThe proposed method is efficient and able to adapt itself to daily work conditions of classical pathology laboratories.
Highlights
Available microscope slide scanners produce whole slide images at various resolutions from histological sections
The proposed method is efficient and able to adapt itself to daily work conditions of classical pathology laboratories
Staining The histological sections were stained according to the Hematoxylin-Erythrosine-Saffron (HES), Periodic Acid Schiff (PAS) and/or immunostained for hormonal receptors (ER and PR), HER2, proliferation markers (Ki-67 and PHH3), vascular marker (CD31), using automatons and standard protocols of the pathology department
Summary
Available microscope slide scanners produce whole slide images at various resolutions from histological sections. Since less than two decades [2], microscope slide scanner devices give access to an image of the whole histological section [3], so-called “Whole Slide Image” (WSI). It allows a quick inspection of the tissue fragment by panning into its entire section surface and by zooming progressively across the continuous magnification scale [4], thanks to a virtual slide. The aim of the present paper is to detail a solution able to give one high resolution large image of the whole histological section of big lesions, called Composite Virtual. A quality control protocol was settled in addition, in order to evaluate the quality of the results more precisely than only visually
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