Abstract
Water and electrolyte balance is regulated in large part by Na/K ATPase. Inhibition of this enzyme leads to cellular depolarization, a rise in extracellular K and intracellular Na, cellular swelling, and has been associated with shock, hypertension, bipolar disorder, schizophrenia, depression and diabetes. Recently, marinobufogenin, a bufodienolide, has been identified in human and rat plasma and is a strong inhibitor of Na/K ATPase. To determine what bufodienolides are in pig, 1000ml of pooled pig plasma and 500ml of urine was extracted using 1× volume ethyl acetate/ 4× volume acetonitrile, dried, de‐conjugated with glucoronidase/sulfatase, and extracted by affinity chromatography (Digibind®, GlaxoSmithKline). Extract was run on Reverse Phase High Pressure Liquid Chromatography (Beckman Coulter). Chromatographic peaks were identified as marinobufogenin, bufalin, resibufogenin and cinobufagen by 1) co‐elution of standards, 2) UV spectrum and 3) mass spectroscopy of collected fractions. These data show that bufodienolides, potent inhibitors of Na/K ATPase, exist in plasma and urine of pig. Supported by the ARMY MRMC.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
