Abstract
The second dissociation constant, pK2, and related thermodynamic quantities for TAPSO have been previously determined and reported from the temperatures (278.15 to 328.15) K. In the current study there are five buffer solutions without NaCl and five buffer solutions with NaCl present which yield an ionic strength (I = 0.16 mol·kg-1) similar to that of blood plasma. These buffer solutions have been evaluated in the temperature range of (278.15 to 328.15) K using the extended Debye-Huckel equation, due to the limitations of the Bates-Guggenheim convention such that it is only valid when I -1. The liquid junction potential (Ej) values between the TAPSO solution and the saturated KCl calomel electrode solution have been estimated at (298.15 and 310.15) K using a flowing junction cell measurement. The previously mentioned Ej values have been used in determining the operational pH values at (298.15 and 310.15) K. These TAPSO buffer solutions are recommended as reference solutions for pH measurements in saline media with an ionic strength of I = 0.16 mol·kg-1.
Highlights
INTRODUCTIONRegarding the current experiment the goal of the authors is to provide staunch pH values for the ampholyte 3-[N-tris(hydroxymethyl)-methylamino]-2-hydroxypropanesulfonic acid (TAPSO) depicted by the following structure: This zwitterionic buffer, among others suggested by Bates and coworkers [5,10], may be used as biological buffers for biological media
Good and his associates [1,2] have suggested several useful zwitterionic amino acid buffer solutions used in measuring the pH of blood and having the pH constrained so it closely reflects that of the physiological range
Regarding the current experiment the goal of the authors is to provide staunch pH values for the ampholyte 3-[N-tris(hydroxymethyl)-methylamino]-2-hydroxypropanesulfonic acid (TAPSO) depicted by the following structure: This zwitterionic buffer, among others suggested by Bates and coworkers [5,10], may be used as biological buffers for biological media
Summary
Regarding the current experiment the goal of the authors is to provide staunch pH values for the ampholyte 3-[N-tris(hydroxymethyl)-methylamino]-2-hydroxypropanesulfonic acid (TAPSO) depicted by the following structure: This zwitterionic buffer, among others suggested by Bates and coworkers [5,10], may be used as biological buffers for biological media. The currently used NBS certified physiological phosphate primary standard buffer has recorded pH values of 7.415 and 7.395 at (298.15 and 310.15) K, respectively [5] This phosphate buffer is comprised of KH2PO4 (0.008695 mol·kg–1) and Na2HPO4 (0.03043 mol·kg–1). The zwitterionic buffer compound TAPSO is not expected to yield such adverse effects, but the potential for complex formation with cations such as Mg2+ and Ca2+ does still exist The likeliness of this occurring has been minimized with a high NaCl: buffer concentration ratio for an isotonic saline solution of I = 0.16 mol·kg–1. The detailed procedure for preparation of the aforementioned TAPSO buffer solutions is described in the Experimental Section below
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