Buffalo Milk Casein Derived Decapeptide (YQEPVLGPVR) Having Bifunctional Anti-inflammatory and Antioxidative Features Under Cellular Milieu

Abstract

Antioxidants having anti-inflammatory potential will be useful in reducing the progression of many lifestyle associated diseases. Under present investigation, buffalo casein derived decapeptide (YQEPVLGPVR) displayed anti-inflammatory response by suppressed (p < 0.01) murine splenocytes proliferation, reduced inflammatory cytokine levels (Interferon-γ) besides elevated levels of regulatory cytokines (Interleukin-10 and Transforming Growth Factor-β) in splenocyte culture supernatant. Decapeptide also improved the phagocytosis (p < 0.01) of peritoneal macrophages. Subsequently, antioxidative feature of the peptide was also identified by efficient (p < 0.01) free radical scavenging using chemical assays (ABTS: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid and ORAC: oxygen radical absorption capacity methods) which was later confirmed for protective action against H2O2 mediated oxidative stress on intestinal epithelial cells by inhibition (p < 0.01) of cellular ROS generation, oxidative products formation along with elevated (p < 0.01) activities of antioxidative enzymes (catalase and glutathione peroxidase). Declined (p < 0.01) mRNA expression of a transcription factor (Nuclear factor erythroid 2-related factor: Nrf-2) involved in redox signalling further established the protective effect of peptide against oxidative stress induced injury.