Abstract

The salinity is one of most common stress factors with devastating effects for wheat ( Triticum aestivum L.) and barley ( Hordeum vulgare L). The aim of this study was to determine the salt stress response associated with WRKY transcription factors by gene expression analysis. Seeds of T. aestivum L. cvs. Esperia and Bezozya, and H. vulgare L. cvs. Lord and Ramata were subjected to salt stress for 10 days with two concentrations of NaCl (1.0 % and 2.0 %). No germination was observed in 2.0 % NaCl treated seeds. Nearly all the WRKY genes studied were upregulated and downregulated in response to 1.0 % NaCl stress in relatively resistant and sensitive cultivars in comparison to control sets, respectively. Among the screened genes, the expression of TaWRKY7 , 40 , 41 , 53 , 68 , 72 and 79 genes were increased in relatively resistant Esperia cultivar in wheat. Similarly, 7 of WRKY genes ( HvWRKY6 , 9 , 24 , 25 , 33 , 34 , 42 ) were upregulated in relatively resistant Lord cultivar in barley. In terms of WRKY gene expression profile, since TaWRKY 7 and TaWRK72 in wheat and HvWRKY33 in barley increased significantly, these genes can be used as marker genes for further investigation of abiotic stress response. This study is a preliminary study in terms of providing an association between WRKY genes and salinity stress response of wheat and barley breeding cultivars in Turkey.

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