Abstract
Lipoxygenase (LOX) activity was detected in buckwheat extracts, and was shown to have a pH optimum of 6·5. LOX activity was not affected during 6 days of germination. The specific activity increased when ground buckwheat seeds were extracted with 0·01% or 0·1% solutions of calcium chloride or magnesium chloride, compared to extractions with distilled water or 1·0% solutions of either of these salts. Increase in specific activity was greater in the presence of calcium ions compared to magnesium ions. Gamma-irradiation of buckwheat seeds up to 4 kGy resulted in a decrease in LOX activity to a level of approximately 22% of the untreated control, but increased to approximately 43% of the control at a dose of 6 kGy. These results suggest that gamma-irradiation reduces LOX activity, but is not likely to completely prevent lipid oxidation in buckwheat.
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