Bubble manipulates the release of viral aerosols in aeration

Abstract

Bubble bursting is a common phenomenon in many industrial and natural processes, plays an important role in mediating mass transfer across the water-air interface. But the interplay between bubbles and pathogens remains unclear and the mechanisms of virus aerosolization by the bubble properties have not been well studied. The main objective of this study was to evaluate the water-to-air transfer of viruses by bubbles of different sizes. Unlike the dominant view of smaller bubbles less bioaerosols, it was found that the smaller bubbles could generate significantly more viral aerosols regardless of the virus species (Phi6, MS2, PhiX174, and T7), when the Sauter mean bubble diameters were between 0.56 and 1.65 mm under constant aeration flow rate. The mechanism studies denied the possibilities of more aerosols or better dispersion of viruses in the aerosols generated by the smaller bubbles. However, deeper bubbling could transfer more viruses to the air for MS2, PhiX174, and T7. Their concentrations in aerosols were linearly related to the bubbling depth for these non-enveloped viruses, which demonstrates the bubble-scavenging effect as a main mechanism except for the enveloped virus Phi6. Whereas, unlike these three non-enveloped viruses, Phi6 could survive relatively better in the aerosols generated from the smaller bubbles, though the enhancement of aerosolization by the smaller bubbles was much larger than the improvement of survival. Other mechanisms still remain unknown for this enveloped virus. This study suggests that the attempt of decreasing the bubble size in aeration tank of the wastewater treatment plant might significantly increase the solubility of oxygen as well as the risk of viral aerosols.