Bubble‐driven solid‐phase microextraction for the rapid determination of potassium sorbate in oyster sauce by HPLC‐DAD

Abstract

SummaryOn‐site sample preparation has the advantage of combining sampling and sample preparation into a single step, thereby minimising sources of error, and eliminating the risk of analyte instability. Bubble‐driven solid‐phase microextraction (BD‐SPME) was first proposed as an on‐site pretreatment method. Oyster sauce underwent on‐site pretreatment to measure potassium sorbate levels via high‐performance liquid chromatography with a diode array detector (HPLC‐DAD), obviating the need for stirrers. The high sensitivity of the HPLC determination of potassium sorbate was obtained using BD‐SPME pretreatment with a limit of detection (LOD) of 0.03 mg L−1 and a limit of quantification (LOQ) of 0.10 mg L−1. The refined technique displayed remarkable linearity from 0.10 to 30.00 mg L−1, boasting a coefficient of determination R2 ≥ 0.998. In addition, the recoveries of potassium sorbate in oyster sauce using the method were in the range of 90.8–101.4%. Evidently, BD‐SPME in combination with HPLC‐DAD is a sensitive and promising analysis for on‐site pretreatment.