Abstract
Objective: To qualitatively investigate whether a prototype brush composed of metal bristles collects oral epithelial cells effectively for cytological evaluation of oral mucosal lesions. Material and Methods: Twenty patients with suspicious oral mucosal lesions were enrolled. Patients were asked to gargle with saline and to deposit the oral rinse into specimen cup. Then, oral mucosal cell samples were collected using a metal oral brush, via sweeping motion. Punch biopsy was performed for histological examination. All samples were evaluated with liquid based cytology (LBC) according to the cellularity, the depth of the epithelial layer, cellular integrity by an oral pathologist. Results: Oral rinse provided samples with 100% cellular integrity and cellularity, mostly from the intermediary layers. With metal brush, both inadequate cellularity and cellular integrity was observed in 25% of the cases. Cellular integrity was adequate in 65%, cellularity was adequate in 45% of the lesions. Samples were dominantly from the intermediary layers, but in one case, metal brush collected cells from the parabasal layer. Conclusion: The narrow spiral pitch and width of metal bristles may have resisted to release the cellular samples collected. With adjustment of the spiral pitch and diameter of metal brush bristles, its’ efficacy could be enhanced.
Highlights
Oral Potentially Malignant Disorders (OPMD) are morphologically altered oral mucosal tissue with higher risk of progression to cancer and usually revealed during clinical examination [13]
Objective: To qualitatively investigate whether a prototype brush composed of metal bristles collects oral epithelial cells effectively for cytological evaluation of oral mucosal lesions
All samples were evaluated with liquid based cytology (LBC) according to the cellularity, the depth of the epithelial layer, cellular integrity by an oral pathologist
Summary
Oral Potentially Malignant Disorders (OPMD) are morphologically altered oral mucosal tissue with higher risk of progression to cancer and usually revealed during clinical examination [13]. The major histological criteria for diagnosis of an epithelial dysplasia are abnormal patterns of keratinization [6], hyperplastic basal cells, enlarged and hyperchromatic nuclei, and drop-shaped rete ridges [7], which may be present at the same site before the malignant changes develop [3,8]. Even though these alterations may be observed within the whole thickness of epithelium, they do not destroy the basement membrane to invade the underlying stromal tissues [6]. OPMD might turn into carcinoma in a percentage varying between 5-18% [11,12], but the presence of moderate or severe dysplasia has been accepted to have the highest risk for malignant transformation [13,14], ranging from 11-36% with a mean time of 33.6 months [13]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Pesquisa Brasileira em Odontopediatria e Clínica Integrada
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
