Brucellamelitensis outer membrane protein 25 interacts with ferritin heavy polypeptide 1 in human trophoblast cells.

Abstract

Outer membrane protein 25 (OMP25) is involved in Brucella virulence and serves an important role in Brucella pathogenesis during infection. However, the protein interacting with OMP25 in host cells remains to be elucidated. The present study constructed a cDNA library from Brucellamelitensis 16M‑infected human placenta trophoblastic cells (HPT‑8) and identified and confirmed the interaction between Brucella OMP25 and ferritin heavy polypeptide1(FTH1) of HPT‑8 using yeast two‑hybrid and co‑immunoprecipitation technologies. The expression of Toll‑like receptor (TLR)4, myeloid differentiation primary response protein MyD88 (MyD88) mRNA and inflammatory factors was detected by RNA interference. The results showed that OMP25 interacted with FTH1. Subsequently, TLR4 and MyD88 mRNA expression levels and inflammatory factors, such as nitric oxide, lactate dehydrogenase and TNF‑α, significantly increased after inserting OMP25 into the HPT‑8 cells. Notably, a low dose of OMP25 resulted in immunological protection, whereas high dose of OMP25 resulted in a cytotoxic effect on the HPT‑8 cells. It is suggested that OMP25 and FTH1 serve important roles in intracellular parasitism of Brucella and inhibition of expression.