Brucella Seroprevalence in a High-Risk Population in Greece: A Cross-Sectional Study

Panagiotis Andriopoulos,Natalia Gioti,Andrea Paola Rojas Gil,Dimitrios Floros,Maria Tsironi,Anargiros Mariolis

doi:10.1155/2018/8751921

Panagiotis Andriopoulos, Natalia Gioti + Show 4 more

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https://doi.org/10.1155/2018/8751921

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Abstract

Introduction Brucellosis is a zoonosis with high occupational risk. However, seroprevalence of Brucella antibodies among occupational groups is not known, since studies in endemic countries are rare. Methods A cross-sectional seroprevalence study was conducted among livestock farmers in an endemic region in Greece. A low-risk group of individuals that just moved in the region was used as controls. Rose Bengal, Wright standard tube agglutination (STA) tests, and specific IgG and IgM antibodies using ELISA were evaluated; differences and odds ratios were calculated. Results were compared with studies from other endemic regions. Results 100 livestock farmers and family members and 34 first-year students were enrolled. Rose Bengal results were 18% positive versus 0% (p=0.007); Wright STAs for Brucella melitensis were 8% versus 2.9% (p=0.448) and for Brucella abortus they were 2% versus 2.9% (p=0.588). ELISA IgG was positive in 8% of farmers versus 2.9% of students (p=0.448). Parallel testing with Rose Bengal and ELISA IgG was positive in 3% versus 0% (p=0.571). No significant odds ratios were calculated for Wright STAs and ELISA IgG. Conclusions Healthy livestock farmers may present with positive Rose Bengal test but this translates to true seroprevalence in only a small proportion. Livestock farmers have no significant seroprevalence that may obscure diagnosis of acute brucellosis in clinical settings.

Highlights

Brucellosis is a zoonosis with high occupational risk
Brucellosis is a disease with occupational interest, since a great percentage of the affected population is livestock farmers, their families, slaughterhouse workers, and veterinarians [9,10,11,12]
The occupationally exposed high-risk population was, as expected, older than the group of students (mean age 57.7 years versus 19.5 years) and predominantly male. 18% of the high-risk group had positive Rose Bengal test, 8% Wright melitensis standard tube agglutination (STA), and 2% abortus STA

Summary

Introduction

Seroprevalence of Brucella antibodies among occupational groups is not known, since studies in endemic countries are rare. A cross-sectional seroprevalence study was conducted among livestock farmers in an endemic region in Greece. Rose Bengal, Wright standard tube agglutination (STA) tests, and specific IgG and IgM antibodies using ELISA were evaluated; differences and odds ratios were calculated. Parallel testing with Rose Bengal and ELISA IgG was positive in 3% versus 0% (p=0.571). Healthy livestock farmers may present with positive Rose Bengal test but this translates to true seroprevalence in only a small proportion. Livestock farmers have no significant seroprevalence that may obscure diagnosis of acute brucellosis in clinical settings. Brucellosis is a disease with occupational interest, since a great percentage of the affected population is livestock farmers, their families, slaughterhouse workers, and veterinarians [9,10,11,12]. Individual specific IgG and IgM immunoglobulins can be measured rapidly but at a much greater cost than simple agglutination tests [21]

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