Brucella abortus Detection by Indirect Competitive ELISA and Rose-bengal tests in Stray Dogs of Some Rural Areas in Wasit,Al-Qadisiyah and Dhi-QarProvinces / Iraq

Abstract

Brucella abortus is a gram-negative bacterium afflicting,mainly cattle,but can be attacked the dogs causing brucellosis disease. In some rural areas of three Iraqi provinces (Wasit,Al-Qadisiyah and Dhi-Qar),the stray dogs submitted for two an effective serological tests (rose-bengal test and indirect competitive ELISA) to detect an existence of the specific IgG antibodies against B. abortus. The results of this study reported (28.89%) and (39.26%) as a total infection rate by rose-bengal and ELISA test,respectively. According to studied areas,the seropositive infection rates by rose-bengal and ELISA tests in (Wasit,Al-Qadisiyah and Dhi-Qar provinces) were (31.25% and 45.83%), (38.71% and 54.84%); and (21.43% and 25%),respectively. The significant differences were reported between and within every examined province at level (P˂0.05).