Abstract
A fungus isolated from naturally brown-stained red pine sapwood was found to produce characteristic chocolate brown stain in sterilized red pine sapwood in culture; it is identified as a species of Cytospora. The fungus develops in the ray parenchyma, may proliferate in the tracheids, but penetrates the walls only through the pits. A technique is described for producing brown stain in culture in sticks of the size required for static bending and toughness tests. Two series of sticks were subjected to each test; the samples used were end-matched sticks in groups of three, which provided a green control, sterilized control, and specimen stained in culture by Cytospora sp. The results showed some variation in the relative strength of samples in different groups, but a statistical analysis indicated that the effect of Cytospora sp. on both bending strength and toughness is negligible.
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