Abstract
A mild, specific chemical procedure suitable for large number of samples has been developed for the decolorization of sulfonphthalein dyes. The method is based on electrophilic bromination of the dye using aqueous N-bromosuccinimide with concomitant destruction of the sultone ring and elimination of sulfur trioxide. Reduction of the presumed quinoid intermediate with aqueous sodium borohydride completes the process. The method has been applied to the preparation of samples containing 14C and 3H which were too highly quenched by the dye to be counted without its removal.
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