Bromodeoxyuridine labeling reveals a class of satellite-like cells within the electric organ.

Abstract

When the electric organ (EO) of weakly electric fish is amputated, a blastema forms from which new EO and muscle cells arise. However, the progenitor cells that contribute to the blastema are unknown. We studied regeneration of the electric organ in Sternopygus to answer this question. The EO of this species is composed of electrocyte cells surrounded by peripheral bundles of muscle fibers. Fish were injected with 5'-bromodeoxyuridine (BrdU) 24 h after amputating the terminal portion of the EO. At this time, a population of small cells were labeled in the extracellular matrix between electrocytes and muscle fibers. These cells did not label in control fish injected with saline or in nonamputated BrdU-injected fish. For the first 6 days postamputation, increasing numbers of BrdU-labeled cells appeared at the wound margin. A blastema formed 6 days after amputation and contained numerous BrdU-labeled cells. At 10 days postamputation, clusters of BrdU-positive cells were seen throughout the wound margin and proximal blastema. At 14 days, BrdU-labeled nuclei were present within developing electrocytes. Labeling alternate sections with MF20 antimyosin and AE1 anticytokeratin antibodies confirmed that BrdU-positive multinucleate cells coexpress myosin and cytokeratin epitopes, diagnostic of newly regenerated electrocytes. Electron micrographs reveal that the small cells surrounding muscles and electrocytes are similar; they contain an elongate nucleus, are largely devoid of cytoplasm, and possess few organelles. This morphology and evidence of myogenic potential suggests that these cells are satellite cells.