Broccoli bioactives inhibit human prostate cancer cell invasions in vitro (1045.5)

Abstract

Previous studies have found that various bioactives in broccoli may have anti‐cancer activities, including anti‐invasion ability. We hypothesized that regular consumption of broccoli may inhibit prostate cancer via inhibition of invasion. Thus, the invasive human prostate cancer cell line, DU145, was treated with two broccoli bioactives—sulforaphane and indole‐3‐carbinol. Cells were seeded in matrigel inserts in 24‐well plates containing complete medium as the chemoattractant. Treatments were renewed at the 24th hour. After a total of 48 hours treatment, the matrigel layer was removed, and cells on the bottom membrane were stained and counted. The invasive ability of DU145 was significantly suppressed in a dose‐dependent manner by treatment with sulforaphane at concentrations as low as 3µM. Indole‐3‐carbinol also significantly reduced invasion, but at concentration as low as 0.2µM. In summary, two major bioactives in broccoli show anti‐invasion activity in vitro, at physiologically‐achievable concentrations.