Abstract
ABSTRACT Fifteen New Zealand adult rabbits were randomly allocated into three groups: Sham-operated (group A), Ischemia and Reperfusion (group B) and Carolina Rinse Solution (CRS) (group C). Groups B and C were subjected to one hour of ischemia and two hours of reperfusion. In group C, ten minutes before reperfusion, the bowel lumen was filled with CRS, and the segment immersed in CRS. Necrosis and loss of integrity of the villi were visible in groups B and C. Edema of the submucosa and circular muscle was observed in all groups. Hemorrhage was observed in different layers for groups B and C, but group C showed more severe hemorrhage in different layers during reperfusion. All groups showed polymorphonuclear leukocyte infiltration on the base of the mucosa, submucosa, and longitudinal muscle, in addition to polymorphonuclear leukocytes margination in the mucosal and submucosal vessels. Necrosis of enterocytes, muscles, crypts of Lieberkühn and myenteric plexus was observed in groups B and C during reperfusion. Topical and intraluminal Carolina Rinse Solution did not attenuate the effects of ischemia and reperfusion in the small intestine of rabbits.
Highlights
Obstruction of blood vessels leads to ischemia and reduction of oxygenation and tissue perfusion (Moore et al, 1995)
This study has investigated the effects of topical and intraluminal Carolina Rinse Solution (CRS) in a rabbit model of jejunal ischemia and reperfusion (I/R)
Diffused necrosis of enterocytes was observed in groups B and C within five minutes of reperfusion and, after two hours of reperfusion, both groups showed similar shortening of the villus
Summary
Obstruction of blood vessels leads to ischemia and reduction of oxygenation and tissue perfusion (Moore et al, 1995). Ischemia causes tissue necrosis unless blood flow returns in a timely fashion. Anesthesia was induced with 15 to 25mg/kg of sodium thiopental (Thipentax®, Cristália Produtos Químicos e Farmacêuticos Ltda., Brazil), intravenously. After injecting 5ml of 2% lidocaine hydrochloride (Xylestesin® Cristália Produtos Químicos e Farmacêuticos Ltda.) subcutaneously, a sevencentimeter midline incision was performed caudally from the xiphoid process, exposing the bowel. A ten-centimeter segment of distal jejunum was chosen for the study. Both the mesenteric artery and vein were isolated using a 0.2-mm nylon monofilament fishing line and brought together into a 2-cm section of polyvinyl urethral tube No 4 in order to establish ischemia of the segment. After one hour of ischemia, the sutures were removed, and the isolated segment remained in reperfusion for two hours. At the end of the experiment, the animals were euthanized with a lethal dose of thiopental
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