Abstract
Objective To investigate the effect of breviscapine (BVP) on the development of prostate cancer and its molecular mechanism. Materials and Methods After treatment with breviscapine and microRNA-129-5p, MTT (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) and cell counting kit-8 (CCK-8) tests were performed to examine the proliferation rate of cells, while Transwell was used to analyze cell migration ability; at the same time, quantitative real-time polymerase chain reaction (qRT-PCR) was applied to detect the expression of microRNA-129-5p and ZFP91 in prostate cancer cells. In addition, the binding of microRNA-129-5p and ZFP91 was confirmed by dual-luciferase reporting assay; meanwhile, cell reverse experiment verified that breviscapine can regulate ZFP91 via upregulating microRNA-129-5p. Results The results of MTT, CCK-8, and Transwell experiments demonstrated that breviscapine inhibited the proliferation as well as the migration capacities of PC cells; meanwhile, it upregulated the level of microRNA-129-5p in PC cells while downregulated that of ZFP91. Furthermore, dual-luciferase reporter gene assay verified that ZFP91 was a potential target of microRNA-129-5p. Finally, cell reverse experiment confirmed that breviscapine downregulated ZFP91 expression by upregulating microRNA-129-5p, while downregulation of microRNA-129-5p partially reversed the inhibitory effect of breviscapine on cell proliferation ability. Conclusions Breviscapine may inhibit the expression of ZFP91 through upregulating microRNA-129-5p and thus participating in the progression of PC.
Highlights
Natural existing compounds are regarded as the most promising drugs for the prevention and treatment of cancer.ey have various model effects and limited toxicity and can regulate cell proliferation and cell cycle arrest [1,2,3]
In order to detect whether breviscapine has an effect on Prostate cancer (PC) cell proliferation, cells were administered with different concentrations of breviscapine, and MTT assay was performed 24 hours later
We carried out the subsequent cell counting kit-8 (CCK-8) and Transwell experiments, which showed that breviscapine markedly inhibited the proliferative and migrant ability of LNCap and PC3 cells (Figures 1(b) and 1(c)). ese results demonstrated that breviscapine inhibits the proliferative and migrant ability of PC cells
Summary
Natural existing compounds are regarded as the most promising drugs for the prevention and treatment of cancer.ey have various model effects and limited toxicity and can regulate cell proliferation and cell cycle arrest [1,2,3]. Natural existing compounds are regarded as the most promising drugs for the prevention and treatment of cancer. Studies have found that BVP can be used to induce apoptosis and inhibit cell proliferation, preventing the progress of various tumors [6]. MiRNA can regulate various biological processes such as cell proliferation, differentiation, and apoptosis [7]. Abnormal miRNA expression has been found in various human tumor tissues including prostate cancer tissues [8,9,10]. Abnormal expression of some miRNAs has been proved to be closely related to the drug resistance and metastasis of prostate cancer [11,12,13]. Ese miRNAs can be used as biomarkers for predicting the progress of prostate cancer. The level of microRNA-141 in serum of patients
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