Brevetoxin Preservation in Marine Sediments from Amber Jack Blue Hole

Abstract

In Southwest Florida (SWFL) red tide (Karenia brevis) is a natural hazard and produces potent toxins, to both human and marine life, known as brevetoxins. Red tide, a microscopic alga species, is responsible for environmental, economic, and human health problems in this region. However, our understanding of natural and anthropogenic drivers of red tide are still poorly understood. This study endeavors to reconstruct brevtoxins records in marine sediments with the purpose to better understand present day blooms compared to historic red tide blooms in SWFL. This may be best achieved by looking at sediment cores from blue holes along the West Florida shelf. Blue Holes are natural geologic formations originating on land, as sink holes, which filled over time as global sea level rose. Generally, anoxic conditions found in blue holes provide excellent preservation of long geologic records and provide a unique opportunity to study brevetoxin as biomarkers in sediment cores. In 2023, sediment cores were collected from the Amber Jack Blue Hole off the coast of SWFL. The sediment cores were sliced at 1 cm intervals, and solvent extracted for the analysis of brevetoxins using a LC-MS/MS. The sediments were also analyzed for grain size analysis and scanned using x-ray. This study examines the vertical preservation depth of Karenia brevis and analyzes historic red tide events preserved within the Amber Jack blue hole sediments. This research provides the first opportunity to study red tide chemical biomarkers in high resolution blue hole sediment cores.