Breeding strains of Panax notoginseng by using EST-SSR markers

Abstract

To comparatively determine the genetic variation and differentiation of different breeding strains of Panax notoginseng for providing the basic information for genetic breeding. The genetic diversity and genetic structure of the 17 breeding strains of P. notoginseng were assayed by using EST-SSR molecular marker. A total of 136 polymorphic loci of EST-SSR were detected in the 17 breeding strains of P. notoginseng, with the PIC (polymorphism information content) being 0.78, H (the gene diversity within population) being 0.139, the I (the Shannon's information index) being 0.208. Gst (coefficient of gene differentiation) was 0.382 among the 17 strains. The cluster analysis of genetic similarity showed that the 17 strains of P. notoginseng and P. stipuleanatus were classified into 4 groups, while the 17 strains of P. notoginseng were classified into three subgroups. The genetic differentiation was detected among the 17 strains of P. notoginseng from the same cultivation population by bulk selecting. And it was feasible to detect the effect of bulk selection by EST-SSR markers.