Breeding Improvement of Laurus nobilis L. by Conventional and In Vitro Propagation Techniques

Abstract

SUMMARY Originating from Asia Minor and later transmitted through Greece, Laurus nobilis L. (Lauraceae) has overrun Europe and the Mediterranean area. The problems associated with the great variability of this dioïceous species, the difficulties in sexual reproduction and seed germination, could be overcome, using micropropagation and in vitro culture techniques, by mass clonal production, as was demonstrated in our laboratory with other species. Here we present preliminary results of the breeding improvement and micropropagation by axillary buds of selected mature individuals. An analysis of variance of the results showed significant differences in rooting due to the type of cutting. The buds also have an effect in this morphogenetic process, especially in the induction of the first root. In micropropagation trials, we could control the delicate stage of sterilization. Aseptic and vigorous cultures were obtained after treatment by calcium hypochlorite. Shoot multiplication and elongation were obtained by addition of benzyl-aminopurine combined with gibberellic acid. Root induction was obtained in MS medium salts at ⅓ strength with naphtalene-acetic acid.