Breast Milk from Frequent Trans Fatty Acid Consumers Shows High Triglyceride and Glucose Levels, but Low Cholesterol and Apolipoprotein A-I levels, with Resulting Impaired In Vitro Zebrafish Embryo Growth and Survival.

Abstract

It is well known that breast milk is the best nutritional source for infant growth. However, there has been no information about the quality of breast milk from individuals who daily consume a trans fatty acid (TFA)-enriched diet. We performed compositional and functional analyses with breast milk from lactating mothers, in terms of lipid content and zebrafish embryo survivability, among individuals who daily consumed TFA-enriched food (n = 5), normal diet as control (n = 5), and powder formula (n = 5). In lipid content of breast milk, the control group showed 2.5- and 4.5-fold higher cholesterol content than the TFA group and infant formula, respectively. The TFA group and infant formula showed 1.8- and 2.0-fold higher triglyceride (TG) than the control group. Moreover, the TFA group and formula showed 1.4- and 4.8-fold higher glucose levels compared with control. The TFA group also showed 25% lower protein content than control. Microinjection with breast milk (50 nL) from the TFA group showed significantly lower zebrafish embryo survivability (50% ± 4%) compared with the control (66% ± 5%), whereas microinjection with formula showed the lowest survivability (39% ± 5%) with the slowest developmental speed. Immunodetection revealed that breast milk from the TFA group showed smaller-sized apoA-I (25.5 ± 0.6 kDa) than that from the control group (27.5 ± 1.5 kDa), whereas formula did not contain apoA-I. Larger apoA-I size in breast milk was directly associated with higher embryo survivability. Breast milk from the TFA group showed increased TG and loss of cholesterol, lactalbumin (14 kDa), and apoA-I proteins, resulting in functional impairment of development and growth.