Abstract
Objectives: The present study undertaken to explore antioxidant and the cell line study of isolated compound from ethanol extract from Tinospora cordifolia belongs to the family Menispermaceae.
 Methods: The air dried powdered sample of aerial parts of T. cordifolia was extracted in a Soxhlet using five different solvents. Most active ethanol extracts were purified using silica gel column chromatography. Characterized the structure of the isolated compound using Fourier transform infrared spectrum, 1H nuclear magnetic resonance, and liquid chromatography–mass spectrometry studies. Antioxidant and anticancer activity of isolated compound was determined using 2,2-diphenyl-1-picrylhydrazyl and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
 Results: Magnoflorine was isolated from most active ethanol extract from aerial parts of T. cordifolia which shows maximum antioxidant activity 64% at the tested concentration 500 μg/ml. The percentage of cell viability varied from 53.3% at the minimum tested concentration 3.12 μg/ml to 1.9% at the maximum tested concentration 100 μg/ml.
 Conclusion: The isolated characterized compounds would be useful to prepare plant-based pharmaceutical preparation to treat various diseases linked with human diseases.
Highlights
Novel chemical compounds are synthesized from the plant active constituents, which are used in medicine and other useful applications [1]
Plant-based drug discovery has contributed and is contributing to the development of anticancer drugs as well as new anticancer lead compounds in clinical trials [8]
Isolation of bioactive compound from ethanol extract of T. cordifolia aerial Five grams of ethanol extract of aerial part of T. cordifolia were loaded into a column packed with a solution of silica gel (60–120 mesh) with DCM using wet slurry method
Summary
Novel chemical compounds are synthesized from the plant active constituents, which are used in medicine and other useful applications [1]. Purification and isolation of bioactive compounds from plant is a technique that has undergone new development in recent years [2,3]. Carotenoids are mainly synthesized by plants and microorganisms but not animals. These are important dietary sources of Vitamin A and are considered valuable in preventing human diseases [5]. Plant-based drug discovery has contributed and is contributing to the development of anticancer drugs as well as new anticancer lead compounds in clinical trials [8]
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