Abstract
Successful development of safe and highly effective nanoprobes for targeted imaging of in vivo early gastric cancer is a great challenge. Herein, we choose the CdSe/ZnS (core-shell) quantum dots (QDs) as prototypical materials, synthesized one kind of a new amphiphilic polymer including dentate-like alkyl chains and multiple carboxyl groups, and then used the prepared amphiphilic polymer to modify QDs. The resultant amphiphilic polymer engineered QDs (PQDs) were conjugated with BRCAA1 and Her2 monoclonal antibody, and prepared BRCAA1 antibody- and Her2 antibody-conjugated QDs were used for in vitro MGC803 cell labeling and in vivo targeted imaging of gastric cancer cells. Results showed that the PQDs exhibited good water solubility, strong photoluminescence (PL) intensity, and good biocompatibility. BRCAA1 antibody- and Her2 antibody-conjugated QD nanoprobes successfully realized targeted imaging of in vivo gastric cancer MGC803 cells. In conclusion, BRCAA1 antibody- and Her2 antibody-conjugated PQDs have great potential in applications such as single cell labeling and in vivo tracking, and targeted imaging and therapeutic effects' evaluation of in vivo early gastric cancer cells in the near future.
Highlights
Gastric cancer is the second most common cancer and the third leading cause of cancer-related death in China [1,2]
Characterization of synthesized CdSe, CdSe/ZnS quantum dots (QDs), and polymer engineered QDs (PQDs) Different from our previous reports [3,32], the liquid paraffin and HDA were used as organic cosolvent to prepare the core CdSe QDs in this study
The existence of lattice planes on the high-resolution transmission electron microscopy (HRTEM) confirms the good crystallinity of the CdSe/ZnS core-shell structure
Summary
Gastric cancer is the second most common cancer and the third leading cause of cancer-related death in China [1,2]. Quantum dots have been used to study the interaction between protein molecules or to detect the dynamic course of signal transduction in live cells by fluorescence resonance energy transfer (FRET) [18,19]. These synthesized quantum dots have significant advantages over traditional fluorescent dyes, including better stability, stronger fluorescence intensity, and different colors, which are adjusted by controlling the size of the dots [20]. Some studies showed that some kinds of quantum dots exhibited toxic effects such as cytotoxicity, tissue toxicity, and in vivo residues [21,22]. How to develop safe quantum dots has become the concern of many scientists
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